Deoxythymidine nucleotide metabolism in Bacillus subtilis W23 infected with bacteriophage SP1Oc: preliminary evidence that dTMP in SP10c DNA is synthesized by a novel, bacteriophage-specific mechanism
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چکیده
منابع مشابه
Evidence that Bacillus subtilis bacteriophage SP02 is temperate and heteroimmune to bacteriophage phi-105.
Some characteristics of Bacillus subtilis phage SPO2 which show that it is a temperate phage are presented. Wild-type SPO2 forms turbid plaques, similar to those of other temperate phages. SPO2 lysogenic strains which are resistant to SPO2 can be isolated; these strains remain stable lysogens despite the fact that they can no longer adsorb SPO2. SPO2 lysogenic strains can be grown for many gene...
متن کاملTransduction in Bacillus subtilis by bacteriophage SPP1.
Lysates of the virulent bacteriophage SPP1 were shown to be capable of mediating generalized transduction. Suppressible mutants of this bacteriophage (sus) were capable of transduction at a lower multiplicity of infection than virulent SPP1. Linkage analysis demonstrated that bacteriophage SPP1 transduced segments of the genome equal in size to that transferred by SP10. This bacteriophage shoul...
متن کاملNucleic acid synthesis in bacteriophage SPO2c 1 -infected Bacillus subtilis.
The synthesis of host macromolecules was shut off very slowly and incompletely by bacteriophage SPO2c(1). No change in the rate of incorporation of radioactive precursors into protein and ribonucleic acid (RNA) could be detected after infection, and the rate of incorporation of thymidine was increased only slightly. The relative proportions of phage and host species of nucleic acids at various ...
متن کاملRevised restriction maps of Bacillus subtilis bacteriophage phi 105 DNA.
Physical mapping of Bacillus subtilis temperate phage phi 105 DNA was carried out by using restriction endonucleases EcoRI, SmaI, and KpnI, and a new revised EcoRI cleavage map is presented. In addition, the EcoRI cleavage maps of six specialized transducing phages carrying sporulation genes of B. subtilis were revised.
متن کاملNucleotide sequence of the temperate Bacillus subtilis bacteriophage SPO2 DNA polymerase gene L.
Temperate Bacillus subtilis phage SPO2 codes for a phage-specific DNA polymerase. The polymerase gene has been cloned, and its nucleotide sequence has been determined. Within the sequence there is an open reading frame starting with a TTG and ending with three consecutive translational stop codons. Ten base pairs upstream from the proposed TTG initiation codon there is a probable ribosome-bindi...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.29.1.61-68.1979